Purpose: Bacterial infections in the lower respiratory tract especially due to Gram negative bacilli, has remained a major complication of endotracheal intubation in patients requiring ventilator equipments. The aim of present cross sectional study was to determine the presence or absence of bacterial infections in tracheal tubes and determination of their antimicrobial susceptibility pattern. Method: A total of 150 inpatients were included. The Endotracheal tubes were vortexed and semiquantitative culture was performed. Inoculation was done on 5% Sheep blood agar and Mac-conkey's agar media, followed by overnight aerobic incubation at 37°C. Phenotypic confirmation and resistotyping of the isolates were done by Vitek-2 AES following CLSI 2014 interpretation criteria. Results: Out of total 150 collected samples, 120 were culture positive (80%). Out of total 120 culture positive cases, in 30 cases, multiple bacteria were isolated (25% of culture positive cases). 56 Klebsiella pneumoniae, 25 Enterobacter aerogenes, 19 Acinetobacter baumannii complex, 8 Pseudomonas aeruginosa and 12 Staphylococcus aureus were isolated. Among total 118 isolates, 40 were Extended spectrum beta lactamase producer(ESBL) (33.89%) and it was comprised of 26 Klesiella pneumoniae and 14 Enterobacter aerogenes. 39 were Carbapenemase producers among whom 19 were Klebsiella pneumoniae, 14 Acinetobacter baumannii and 6 Pseudomonas aeruginosa. All of the Gram negative isolates were sensitive to Polymyxin-B, Colistin and Tigecycline. Among 12 isolated S. aureus, 8 (66.67%) were Methicillin resistant (MRSA). None of the Gram positive isolate was resistant to Vancomycin or Linezolid. Conclusion: This study indicates the emergence of multidrug resistance in a tertiary care health set up
