Gold nanoparticles (AuNPs) have been synthesized by single-step green method using turnip seed extract (TCE) as reducing agent. The synthesized AuNPs were characterized by UV-Vis Spectrophotometer, Transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy and Electron diffraction pattern (EDX). TEM studies revealed the spherical shape and size of gold nanoparticles ranging from 10 to 45 nm. The XRD patterns confirmed the presence of crystalline nature of AuNPs. FTIR measurements confirmed the coating of amine groups, ortho-substituted aromatic phytoconstituents and phenolic compounds on the AuNPs indicating a possible role of biomolecules for the capping and efficient stabilization of the AuNPs. This process of nanoparticle synthesis is simple, nontoxic and eco-friendly compared to the synthetic routes