In the past two decades, many healthcare organizations across the globe have realized the importance of human resources and have shown great concern towards their internal customers by investing in a big way for their growth and development. The present study focuses on Non-clinical employees that are small in number, but plays a key role in the healthcare delivery services. By using Stratified Random Sampling technique, 398 questionnaires were received from Non-clinical employees belong to eight cities of India. The results indicated that there are significant differences in intrinsic and extrinsic motivation level of the Non-clinical employees of India across the eight cities of India. Geographical areas have diverse economic-social-political-cultural factors which affect motivation level.