The study was determined the total infection rate of Toxocara canis 52% in the domestic and stray dogs(42.4% , 65.5%) respectively; at 215 fecal samples were collected from five different areas of Baghdad city that distributed as north, south, west, east and center, from March to October 2015. Statistical analyses appeared that no significant differences p>0.01 among the areas of Baghdad city, no significant differences p>0.01 between sexes of dogs, there are significant differences p <0.01 between the ages of dogs that little puppies more sensitive for infection; and no significant differences p>0.05 among stray dogs. Solid tissue and protocol procedure was applied to T. canis adult worm due to isolate a pure DNA. The result of DNA extraction showed that fresh tissue of adult worm yielded enough DNA concentration for PCR amplification that 100 ng with purity 1.6. The PCR was used for amplify the target fragment by using specific primers were designed manually in the T. canis specific part of ITS-2 Sequences. This study reported the development of sensitive and specific PCR assay allowing rapid and reliable identification of T. canis by the fragment size amplified was 380 bp in ITS-2 gene
