LabVIEW and the signal processing-related toolkits can provide a robust and efficient environment and tools for resolving ECG signal processing problem. This paper demonstrate how to use these advance powerful tools in denoising, analyzing, and extracting ECG signals easily and conveniently not only in heart illness diagnosis but also in ECG signal processing research. Data is imported from online data bank files, such as Physio bank MIT-BIH database to the applications in this kit for analysis. The proposed method deals with the study and analysis of ECG signal using LabVIEW Biomedical toolkit effectively. In the first phase, ECG signal is acquired which is then followed by filtering the raw ECG signal to remove unwanted noises. The next phase focuses on extracting the features from the acquired signal and at last visualizing and analyzing the extraction results.