Aim: To evaluate the effect of non - alcoholic mouth rinses on microhardness of glass ionomer cement, resin modified glass ionomer cement and composite resin. Materials and methods: Total 90 samples were prepared, each group comprised of 30 samples of each group. 30 Glass ionomer samples, 30 resin modified glass ionomer cement samples and 30 composite resin samples were prepared in molds of 3 mm in diameter and 3mm in thickness. All the prepared specimens were stored in artificial saliva for 24 hours to simulate oral environment. Then, 10 samples from each group were immersed in Group I: Miswak mouthrinse (alcohol free), Group II: Colgate plax (alcohol free) and Group III: Artificial saliva (control) for 24 hours at 37 degree celsius. Each specimen will be examined under a Nanointender for evaluating micro hardness. Results: Statistical analysis was done using one-way ANOVA followed by Student ‘t’ test. Group I showed statistically significant difference when compared to Group II and Group III. Conclusion: Group I Alcohol free Miswak rinse showed statistically significant reduction in microhardness, when compared with Group II(colgate plax alcohol free) and Group III (Artificial saliva-control).
