Present investigation deals with an evaluation of phytochemical and antibacterial potential of Gnidia glauca (FRESEN) GILG.. Ethanol, petroleum ether and water were used for preparation of test extracts. The Gnidia glauca were found rich source of phytochemicals like alkaloids, saponin, steroids, tannin, coumarin, flavonoids, diterpenes, cardial glycosides, phenols and phytosterol. Among the extracts ethanolic extract of flowers of Gnidia glauca found to be rich in secondary metabolites after the aqueous once. An active antibacterial compounds were observed in ethanolic extract which shown significant antibacterial activity. The petroleum ether extract of flowers found most efficient. The study provides the surety about the use of Gnidia glauca in drug designing and antibiotics development.