The aim of the present experiment was to study the impact of different concentrations of untreated, primary treated and secondary treated Combined Brewery-Distillery Effluent (CBDE) on various parameters of seed germination and growth, chlorophyll and carotenoid content of Cicer arietinum L. variety GNG26054. The 100% untreated effluent showed very low pH (3.51) and very high COD (5280 mg L-1 ). The study showed the maximum values of positive germination parameters viz. speed of germination (8), peak value (20), germination index (114.5), percent germination (80) and minimum values of negative germination parameters viz. delay index (3) and percent inhibition (0) at 25% untreated effluent. The maximum values of root length (8.2 cm) and shoot length (12.83 cm) were found at 25% primary treated CBDE whereas, minimum values for root length (0.66 cm) and shoot length (1.5 cm) were found at 50% untreated CBDE. Fresh and dry seedling weight also followed the above trend. The chlorophyll a and b, total chlorophyll and carotenoid content showed a gradual decline with increase in effluent concentration with maximum value at 25% primary treated CBDE.
