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Sesame (Sesamumindicum L.) is a flowering plant belonging to the family Pedaliaceae.The present study was carried out to study the effect of different bio-fertilizers on the growth and yield of sesame plant. The bio-fertilizers used were Azospirillum, panchagavya, VAM fungi and a mixture of these three fertilizers. Control plant was maintained without any fertilizer application. In Sesamum, higher germination percentage was observed in seeds soaked in the mixture of bio-fertilizers. The shoot length was found to be more in T3 on the 30th day and T1 on the 75th day. The root length was more in T4 on the 30th day and 45th day. On the 75th day of growth, the root length was higher in T1. The fresh weight and dry weight werefound to be more in T1 on the 75 thday. The number of fruits formed was higher in VAM treated plants on the 75thday.