A non-experimental descriptive study was done using descriptive survey approach to assess the knowledge and practices of food handlers regarding food hygiene in selected nurses hostels at Bangalore south, with the objective of assessing the knowledge and practices of food handlers regarding food hygiene and to find out correlation between their knowledge and practices. The 50 food handlers who satisfied the inclusion criteria were selected by non-probability convenient sampling technique. The structured interview schedule was prepared to find out the level of knowledge regarding concept about food hygiene, food handlers, cooking practices, preservation, and serving and observation checklist for observing the practices related to personal hygiene, habits while cooking , food hygiene, waste disposal, pest control, kitchen infrastructure. The study findings revealed the overall mean knowledge scores of the respondents was found to be 47.8 percent with SD of 11.4 percentage and the overall practice scores on food hygiene was found to be 62.3 percentage with SD of 9.0 percentage there was correlation between food hygiene knowledge and food hygiene practice.