Aim: Isolation and identification of Acinetobacter baumannii in all the clinical samples and to determine antibiotic susceptibility pattern of the isolated. Materials and Methods: This study has been conducted in the Microtech Diagnostic Centre over a period of 24 months from Jan 2013 to Dec 2015. Acinetobacter baumannii and other gram negative were isolated and identified from clinical specimens by standard procedure and antibiotic sensitivity test was performed. Results: The gram negative and Acinetobacter baumannii isolates rate in the present study were 2063 and 116 respectively. Among the clinical samples, Acinetobacter baumannii was highly predominant in respiratory samples. In this current study, different antimicrobials showed higher resistance i.e. 74.1% to ceftazidime, 71.5% to piperacillin, 58.6% to cefepime and lowest resistance was observed to tigecycline (18.1%). All isolates were sensitive to colistin and polymyxin B. Conclusion: Acinetobacter baumannii are now emerging as organisms of nosocomial infections. Hence, antibiotic sensitivity testing and infection control measures are needed to prevent the emergence and spread of multi drug resistant isolates in health care settings
