Aim and objective: To evaluate prevalence of Vitamin D deficiency and establish any correlation between diabetes and vitamin D deficiency among postmenopausal women. Materials and Methods: The 25 – hydroxy vitamin D [25 (OH)D] concentrations were measured by competitive in- vitro quantitative immunoassay. The subjects were classified as vitamin – D- deficient, insufficient or sufficient on the bases of 25 (OH) D concentrations of 30ng /ml respectively. The apparently normal postmenopausal women (PMW) were subjected to fasting between vitamin D deficiency and diabetes. Results: Vitamin D deficiency was observed in 53.35% of the population, 19.48% had insufficiency and 26.83% had adequate Vitamin D levels. In 12.14% of the study population, fasting blood sugar levels were between the normal range which is 70-110 mg/ dl. Correlation between raised blood sugar levels and Vitamin D deficiency among PMW was non-significant (P =0.324). Conclusion:- High prevalence of Vitamin D deficiency exists among apparently healthy. Indian PMW. However the current study failed to show any statistical correlation between Vitamin D deficiency and existence of diabetes, which may be due to small sample size.