1, 4 Dioxane is a highly toxic inflammable substance present in contaminated air, tap water and a large variety of products ranging from shampoos, moisturizers to detergents, toothpaste and cosmetics. Canada and Qatar have even banned cosmetics and shampoos that contain 1, 4 Dioxane. This study aimed to investigate the potential cytotoxic effects of 1, 4 Dioxane on human hepatic cell line (HEP10) following exposure to different concentrations; which are 500 ppm, 2000 ppm, 5000 ppm and 10,000 ppm w3wwfor four different durations of time; 1, 7, 14 and 21 days . Cell viability and apoptosis level was assessed for all plates using comparison between MTT Assay and LDH Assay. Lower concentration of 1,4 dioxane has showed relatively high cell toxicity for LDH Assay which wasn’t witnessed in previous studies. MTT Assay’s reliability is questionable for checking cell viability.
