The present study was aimed to evaluate the organ toxicity and teratogenic effects, if any,respectively in the dams treated with nimesulide in gestation period at different dose levels and in progeny. Thirty six female albino rats of Sprague dawley strain were equally divided into 3 groups and treated as follows. Group 1 served as control, group 2 received nimesulide @ 20 mg/kg body weight and group 3 received nimesulide @ 60 mg/kg body weight via intramuscular route from 7th to 17th day of gestation. In each group, pregnant rats were subjected to caessarian section on 19th day of gestationfor uterine weights with progeny, resorption sites, inborn progeny body weight, litter size, live and dead numbers, male: female progeny numbers, skeletal staining of progeny with Alizarin-Red S, Alcian blue-Alizarin Red S stains and soft tissue developmental anomalies.Thiobarbituric acid reacting substances (TBARS) andreduced glutathione (GSH) were estimated in kidney and liver on 19th day of gestation. Hepatic and renal biomarkers were estimated on 19th day of gestation.There was a significant difference in sero-biochemical profiles of dams and was more evident with nimesulide treated @ 60 mg/kg body weight. Treatment with nimesulide at higher dose induced oxidative stress and tissue damage of liver and kidney as evident from altered biochemistry and histology. It is concluded that nimesulide at 60 mg/kg body weight in pregnant dams showed more significant damage to liver and kidney as compared to the dose of 20 mg/kg body weight and control, while no teratogenicity was reported in any of the tested doses
