Varietal identification or discrimination of cultivars is essential for quality seed production. Seed protein and isozyme electrophoresis have been utilized as a tool for varietal identification. The study was undertaken at the Department of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore in nine mustard genotypes (Rohini, Varuna, Bio 902, PCR 7, Kranti, Pusa Bold, Maya, RN 393 and GM-2).Electrophoresis of seed proteins showed a total of 7 bands in Maya, GM-2, Varuna, PCR 7 and Pusa Bold whereas for rest of the genotypes some bands were absent. The critical bands for identification were the bands at Rf values 0.237, 0.327 and 0.667 which formed the 1st, 3rd and 7th band, respectively. The intensity of the band also varied among all the genotypes. Hence the present study obviously indicated the use of SDS page profile through electrophoresis for discrimination of mustard genotypes and Electrophoresis of seedling peroxidase expressed 7 bands in all the genotypes except in GM-2, PCR 7 and Pusa Bold. The critical band for identification was at Rf value of 0.944 at 7th band It is confirmed that seedling peroxidase could be used effectively to analyze variation among the mustard genotypes.
