The present research paper was projected study water quality status and fish population of Bhagirathi river of Uttarkashi, Uttrakhand. Bhagirathi is the important tributary of holy river Ganga of India, originated from Gomukh Glacier. People of Uttrakashi are facing many problems the scarcity of drinking water, irrigation, generation of electricity in rainy season. In order to make a decision the water quality parameters measurement, use of its for public, recreation, river ecology, fish diversity etc. The parameters like pH, conductivity, and turbidity TDS, DO, BOD, total alkalinity, total hardness, were determined. Higher TDS values were recorded in all sampling site in winter and lower in summer. DO was observed maximum in winter and minimum in June to September. BOD was observed high in monsoon and low in winter. It was concluded that good water quality condition in winter, while at summer and rainy season showed poor water quality. The results give good advice that urgent need to for proper water management in rainy season and the suitable tools to restore the water quality of this river for a healthy and promising human society and river ecology for conservation of fish habitat, fishery development etc.